China Pharmacist 2017;20(3):409-412

UPLC Fingerprint of the Root of Rosa Laevigata Michx from Different Regions

Yulu LI ; Guoxu MA ; Jingquan YUAN ; Wen ZHANG ; Jieming ZOU ; Xiaoqing ZHONG ; Yanlin ZHOU ; Zuolin SU ; Nailiang ZHU ; Xudong XU ; Wei YI

Keywords

Roots of Rosa laevigata Michx; UPLC; Fingerprint; Mutual mode

Country

China

Language

Chinese

Abstract

Objective:To establish the fingerprint analysis method for the root of Rosa laevigata Michx from different regions by UPLC. Methods:The column was ACQUITY UPLC? Phenyl(2.1 mm × 100 mm,1.7 μm). The mobile phase consisted of methanol-water with gradient elution. The flow rate was 0. 2 ml·min-1 , the detection wavelength was 210 nm, the column temperature was 30℃, and the injection volume was 3 μl. Results:The fingerprint consisted of 15 common peaks. The range of similarity for twelve bat-ches of the root of R. laevigata Michx was 0. 489-0. 942. And the reference fingerprint of the root of R. laevigata Michx was estab-lished by UPLC. Conclusion:The fingerprint method is simple and reproducible, which can provide basis for the quality control and the medicinal resources exploration.