Chinese Journal of Forensic Medicine 2016;31(5):476-479

doi:10.13618/j.issn.1001-5728.2016.05.013

Anomalous genotype observation of 4 multi-copy RM Y-STRs

Wenqiong ZHANG ; Chao XIAO ; Jin YU ; Yunshu JIA ; Daixin HUANG

Keywords

forensic biological evidence; Y-STR; rapidly mutating; anomalous genotype

Country

China

Language

Chinese

Abstract

Objective To observe anomalous genotypes of 4 multi-copy RM Y-STRs in Han population in Hubei province. Methods 252 unrelated male samples were ampliifed using reported and newly designed primers, then detected and analyzed by AB 3130 genetic analyzer. Results A total of 25 anomalous multi-band patterns were observed in 20 samples corresponding to an incident rate of 7.94%. 5 anomalous genotypes were observed in DYF387S1 locus, 15 in DYF399S1, 1 in DYF403S1 and 4 in DYF404S1. Four samples showed extra alleles in more than one locus. Conclusion Anomalous genotype has high incident rates in RM Y-STR markers and requires extensive attention in forensic practice.