Journal of Forensic Medicine 2016;32(5):356-362

doi:10.3969/j.issn.1004-5619.2016.05.010

Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population

Ying ZOU ; Juanjuan GUO ; Qingpeng LI ; Daohong ZUO ; Jinshan LIU ; Yadong GUO ; Jie YAN ; Lagabaiyila ZHA ; Jifeng CAI ; Lingmei LAN

Keywords

forensic genetics; polymorphism,genetic; short tandem repeat sequences; Hunan; Han nationality

Country

China

Language

Chinese

Abstract

ObjectiveTo investigate the genetic polymorphisms of 21 short tandem repeat(STR)loci (D3S1358, D13S317, D7S820, D16S539, Penta E, D2S441, TPOX, TH01, D2S1338, CSF1PO, Penta D, D10S1248, D19S433, vWA, D21S11, D18S51, D6S1043, D8S1179, D5S818, D12S391 and FGA). Methods A total of 560 blood samples were collected from unrelated healthy individuals of Han population in Hunan Province. Chelex-100 extraction method was applied to the extraction of genomic DNA, and an AGCU EX22 Kit and 9700 STR amplification was used in amplification reactions. The products were separated and analyzed on 310 Genetic Analyzer.ResultsA total of 248 alleles were observed, the al-lelic frequencies ranging from 0.001 to 0.518. Observation of genotype distributions for each locus showed no deviations from Hardy-Weinberg equilibrium exceptPentaE(P=0.023). The combined pow-er of discrimination, combined power of exclusion, and combined matching probability of the 21 STR loci were approximately 0.999 999 999 999 999 999 999 999 8, 0.999 999 998, and 1.36×10-25, respectively. ConclusionThe 21 STR loci show high polymorphisms in the Han population, which can provide valu-able data and a theoretical basis for forensic individual identification and paternity testing.