Journal of Bacteriology and Virology  2004;34(2):107-112

Analysis of the Cell Lysate Proteome of a Korean Mycobacterium tuberculosis Isolate K01 with H37Rv and H37Ra Strains.

Sung Weon RYU 1 ; Sang Chan PARK ; Mun Nam BANG ; Sung Sik HAN ; Young Kil PARK ; Sue Nie PARK ; Young Soo SHIM ; Seongman KANG ; Gill Han BAI

Affiliations

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Keywords

M. tuberculosis H37Rv; H37Ra; K01; 2-D electrophoresis; MALDI-MS; Cytosol protein; Proteome

Country

Republic of Korea

Language

English

Abstract

Despite recent economic prosperity, Korea still has high prevalence of tuberculosis. Molecular biologic characterization of Korean Mycobacterium tuberculosis strains might provide a deeper understanding of the forces contributing to the spread of tuberculosis in Korea. Therefore, we analyzed the cell lysate proteome of a representative Korean Mycobacterium tuberculosis isolate (K01) in comparison with laboratory reference strains H37Rv and H37Ra. Seven spots were strongly expressed only in K01 strain compared with M. tuberculosis H37Rv and H37Ra. Through continuous MALDI-MS analysis, these spots were identified as hypothetical protein Rv3849, secreted immunogenic protein Mpt64, Acetyl/propionyl-CoA Carbpxylase (AccD1), alkyl hydroperoxide reductase C (AhpC), N-acetylmuramyl-L-alanine amidase, a putative UDP glucose epimerase, and a transposase. A deeper study of these proteins may provide a clue in the development of effective new anti-tuberculosis vaccines against Korean M. tuberculosis isolates.