Journal of Modern Laboratory Medicine 2016;31(3):98-101

doi:10.3969/j.issn.1671-7414.2016.03.027

Establishment and Application of SYBR Green I Real-Time PCR Assay for Rapid Detection of Hepatitis B Virus DNA

Weina HE ; Dongyue L¨U ; Helu LIU ; Jiehui HAN ; Yue HE ; Peipei LI

Keywords

SYBR Green I real-time PCR; Hepatitis B virus DNA; rapid detection; company product comparation

Country

China

Language

Chinese

Abstract

Objective To develop SYBR Green I real-time PCR assay for detection and identification of Hepatitis B virus. Methods Based on the sequences of Hepatitis B virus gp1 gene,primers were designed.The reaction assay and thermal cyc-ling profile were optimized.The positive standard was from recombinant clone.Both the developed assay and Zhejiang kuake biotechnology company’s assay were applied in 100 patients serum.Results The detection limit was between 5×102 copies/ml to 5×108 copies/ml with a good liner correlation and no cross reaction.The whole process just needed 2.5 h.Comparing with the company products,the sensitivity and specificity of the developed assay were 100% and 92.5% respectively.Con-clusion The established assay is rapid,simple,high sensitivity and specificity.It is not only valuable for the identification of Hepatitis B virus patients,but also provide accurate quantitative analysis for HBV patients.