Chinese Journal of Endemiology 2015;34(1):70-72

doi:10.3760/cma.j.issn.2095-4255.2015.01.018

Amplification of cytochrome C oxidase subunit Ⅰ gene of Brandt's Vole by nested PCR

Zhidong MA ; Zili CHANG ; Fang LIU ; Yanhong HU ; Jianyun LI ; Zhenghua WU ; Zhongbing ZHANG

Keywords

Nested PCR; Brandt's Vole; Cytochrome C oxidase subunit Ⅰ gene

Country

China

Language

Chinese

Abstract

Objective To determine a method for amplification of cytochrome C oxidase subunit Ⅰ (CO Ⅰ) gene of Brandt's vole.Methods 发he Brandt's Voles were captured in Abagaqi Xilingol League Inner Mongolia,and DNA was extracted from liver tissue.CO Ⅰ gene was amplified by nested PCR and sequenced afterwards.Results A band of 657 bp and 1 132 bp was amplified by internal and external PCR primers,respectively,which were consistent with expected sizes.A total of 12 segments of Brandt's Vole CO Ⅰ gene sequences were amplified by PCR and verified by sequencing.一he sequence number was KF182196-KF182207 in GenBank.After gene sequence alignment of the 12 CO Ⅰ gene sequences,it was found that the similarity was 100％,and no base mutation.Conclusion CO Ⅰ gene of Brandt's Vole could be amplified by nested PCR without pseudo gene.