Objective:To do research on quality control and calibration period of medical monitor, to assure that the medical monitor is being its best performance and to provide a stable and consistency medical diagnostic images for doctors.Methods: Based on DICOM and AAPM-TG18 calibration standards and using luminance meter and monitor calibration software, our hospital implemented detection and correction quarterly to nine medical monitors. At the same time we tested the brightness response data of medical monitor continuously for 55 weeks and recorded the various brightness with the change of time, then we did some work in statistic analysis and curve fitting using MATLAB software.Results: The performance parameters of our hospital medical monitors are in AAPM-TG18 specified range and the display characteristics are also consistent with the DICOM standard; The change of brightness quarterly of medical monitor is 0~25cd/m2 which is accorded with the function of DICOM GSDF. Conclusion: In order to ensure the accuracy of the impact of image diagnosis system, it’s reasonable and effective to do medical monitors quarterly testing based on brightness response curve.