Herald of Medicine 2015;(7):938-941
doi:10.3870/yydb.2015.07.026
Determination of Paraquat Adsorbability to Four field Soils of Guangxi Province by High Performance Liquid Chromatography Method
Hua LU ; Zong NING ; Daohai CHENG ; Ying QIU ; Junjie HUANG
Keywords
Paraquat; Chromatography,high performance liquid; Adsorbability; Soil
Country
China
Language
Chinese
Abstract
Objective To study paraquat adsorbability of different field soils in Guangxi province of China. Methods HPLC method was adopted to measure the peak area of paraquat in three different media of four types of soils. Chromatographic column was Kromasil C18 column (4. 6 mm×200 mm, 5 μm); mobile phase was acetonitrile-water (including 0. 03 mol·L-1 sodium heptanesulfonate and 0. 24 mol·L-1 phosphoric acid) at a ratio of 397 (pH adjusted to 2. 0 by triethylamine). Detection wave length was 258 nm; column temperature was 25 ℃; the injection volume was 20 μL; flow rate was 0. 8 mL·min-1 . The peak areas of paraquat before and after being adsorbed were compared to calculate the adsorption rate of paraquat in different soils. Results All tested soil samples possessed the adsorption rate of paraquat over 99. 0%. Conclusion Four common field soils in Guangxi province can be used as temporary effective absorbents for the first-aid of paraquat poisoning.
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