Objective To compare the application effect of vasopressin and oxytocin in intramural myomectomy. Methods 50 in -patients for myomectomy were randomly divided into the observation group and the control group, with 25 cases in each group.By injecting oxytocin around hysteromyoma in the control group,and vasopressin in the observation group,hysteromyoma size,number,operation time,amount of bleeding,postoperative hemoglobin,normal body temperature recovering time,anal exhaust time and hospitalization time were compared in two groups.Results Operation time of the observation group was (86.4 ±1.8)minutes,which of the control group was (112.4 ±1.5)minutes,the difference was statistically significant (t =78.462,P <0.05).Blood loss of the observation group was (68.8 ± 27.6)mL,which of the control group was (130.8 ±37.5)mL,the difference was statistically significant (t =9.415, P <0.05).Postoperative hemoglobin of the observation group was (9.6 ±3.6)g/L,which was higher than (5.4 ± 2.6)g/L of the control group,and the difference was statistically significance (t =9.872,P <0.05).The hysteromyoma size of the two groups showed no significant difference (P >0.05 ).The comparing of normal body temperature recovering time,hospitalization time,anal exhaust time of the two groups were not statistically significant (P >0.05). Conclusion Hemostasis effect was evident after applying vasopressin in myomectomy,which can significantly reduce blood loss,shorten surgery time and reduce hemoglobin changes,it′s better than oxytocin and is worth in clinical application.