Objective Compared study on microcolumn gel test (MGT)and tube anti -globulin test (TAT) in the detection of pregnant women IgG anti A (B)in the application of antibody and evaluate the application value of MGT in the prediction of HDN.Methods Choosed blood samples of 443 cases of O blood type pregnant women whose husband were not O blood type as the research object.Every specimen were tested by MGT method and TAT method,and the data were treated statistically.Results The positive rate of MGT method and TAT method were:30% and 12.5% which had statistical significance(χ2 =15.95,P <0.05).The difference was significant in positive cases titer distribution(t =15.13,P <0.01).Conclusion The micro column gel method is rapid,simple,sensitive and repeatable compared of tube anti -globulin test (TAT).