Military Medical Sciences 2015;(5):348-353

doi:10.7644/j.issn.1674-9960.2015.05.007

Comparative proteomic analysis of Yersinia pestis 201 and 201△pCD1 strains

Hongsheng LIU ; Jingyu GUO ; Erling FENG ; Xiaoyi WANG ; Hengliang WANG ; Yajun SONG ; Li ZHU

Keywords

Yersinia pestis; typeⅢsecretion system; electrophoresis,gel,two-dimensional; protein expression profiles; mass spectrometry

Country

China

Language

Chinese

Abstract

Objective To explore the potential pathogenesis of Yersinia pestis and provide new clues for vaccine development through comparative proteomic analysis of wild-type and pCD1 cured strain of Yersinia pestis 201.Methods Differentially expressed proteins at 26℃ and 37℃ were separated and identified using two-dimensional electrophoresis coupled with mass spectrometry .Results A total of 24 differently expressed proteins were successfully identified from the samples of bacteria grown at 26℃ and 25 proteins at 37℃.Among these, 7 proteins were encoded by pCD 1 plasmid. Conclusion Through comparative proteomic research, we have found that the abundance of several proteins can be dramatically changed when the large plasmid pCD 1 is missing,suggesting that the plasmid can regulate the expression of many genes located in the chromosome .