Objective To apply MINI-VIDAS automated multiparametric analyzer to auxiliary diagnosis of the serviceman in order to evaluate its clinical value.Methods Totally 1 528 servicemen complaining of acute chest pain were divided into group A and group B equally; the ones in group A went through conventional examination, and the ones in group B underwent conventional examination combined with instant examination with MINI-VIDAS automated multiparametric analyzer. The differences between the two groups were compared for the interval from hospitalization to definite diagnosis, diagnosis correctness, hospitalization rate, length of stay, recovery rate, improvement rate and etc, and then statistical analysis was carried out.Results The two groups had significant differences in the interval from hospitalization to definite diagnosis and diagnosis correctness (P<0.05), while no statistical differences in hospitalization time, recovery rate and improvement rate (P>0.05).Conclusion MINI-VIDAS automated multiparametric analyzer can be used for the instant examination of cardiac marker to improve the diagnosis of the acute chest pain, and thus can be popularized in military hospital.