China Pharmacist 2014;(2):308-309

Determination of Norisoboldine in Suoquan Capsules by HPLC

Qing WEN ; Ping NIE ; Ye DING ; Jinfeng ZHENG ; Wenli LI

Keywords

Suoquan capsules; Norisoboldine; HPLC

Country

China

Language

Chinese

Abstract

Objective:To establish an HPLC method for the determination of norisoboldine in Suoquan capsules. Methods: The chromatographic procedure with acetonitrile-water containing 0. 5% folic acid and 0. 1% triethylamine (10∶90) as the mobile phase was carried out on an Ecosil C18 HPLC(150 mm ×4.6 mm,5 μm). The flow rate was at 1.0 ml·min-1, the detection wavelength was 280nm and the column temperature was 30 ℃. Results: The linear range of norisoboldine was within 38. 56-578. 40 ng ( r =0. 999 2), the average recovery of norisoboldine was 99. 9%(RSD=1. 7%, n=6). Conclusion:The method is highly sensitive, re-liable and accurate, and can be applied in the determination of Suoquan capsules.