Objective To monitor the quality of in-use disinfectant,evaluate the causes of exceeding of bacterial content standard,and put forward the corresponding management measures.Methods According to the different management methods,disinfectant specimens were divided into experimental group(n =210,from August 2012 to July 2013)and control group (n=190,from August 2011 to July 2012);in-use disinfectant in experimental group was contained with ground-glass stoppered bottles,in-use disinfectant in control group was contained with stainless bottles and ground-glass stoppered bottles,biological monitor results of two groups were compared.Results Quali-fied rate of biological monitor result in experimental group was 100.00% (201/201 ),and in control group was 90.00%(171/190),there was significant difference between two groups(χ2 =21 .13,P <0.05).Conclusion Impro-ving management method can increase the qualified rate of biological monitoring result of in-use disinfectant,and en-sure the safety and effectiveness of disinfection.