Chinese Journal of Schistosomiasis Control 2014;(3):352-354

Optimization of fermentation conditions of molluscicidal endophyte LL3026 from Buddleia lindleyana

Bangxing HAN ; Jun CHEN

Keywords

Oncomelania hupensis snail; Molluscicidal activity; Endophyte; LL3026; Fermentation condition; Orthogonal experiment

Country

China

Language

Chinese

Abstract

Objective To optimize the fermentation conditions of molluscicidal endophyte LL3026 from Buddleia lindleyana. Method The medium composition and cultivation conditions were optimized by orthogonal and single factor experiments. Re-sults The experiments showed that the conditions of initial pH 3,fermentation temperature 30℃,volume of liquid 100 ml(250 ml Erlenmeyer flask),and 3D-xylitol 0.5 g/L were optimum,and the molluscicidal activity of the fermentation filtrate reached 95%. After three hatches of cultivation,the predicted values were verified by validation experiments. Conclusion Endophyte LL3026 from Buddleia lindleyana has a good molluscicidal activity after the optimization.