Objective To investigate the value of the combined detection of serum human epididymal secretory protein 4(HE4) and carbohydrate antigen 125(CA125) in diagnosing ovarian and uterine tumor .Methods Serum levels of HE4 and CA125 were detected in 112 cases of ovarian cancer ,92 cases of uterine cancer ,145 cases of ovarian benign diseases ,138 cases of uterine adeno-myoma and 56 cases of healthy controls by ELISA and electrochemiluminescence(ECL) immunoassay respectively .Results Serum levels of HE4 and CA125 were (246 .80 ± 52 .81)pmol/L and (439 .20 ± 305 .80)U/L in the ovarian malignant tumor group , (196 .50 ± 38 .15)pmol/L and (463 .80 ± 127 .60) U/mL in the uterine malignant tumor group and (19 .76 ± 16 .45)pmol/L and (15 .67 ± 11 .19)U/mL in the control group ,the differences had statistical significance (P<0 .05) .Serum levels of HE4 and CA125 before operation and in postoperative 2 weeks in the ovarian cancer group and the uterine cancer group had statistical difference (P<0 .05) .Conclusion The combination detection of serum HE4 and CA125 can improve the accuracy and sensitivity in the diag-nosis of pelvic tumors .