Chinese Journal of Organ Transplantation 2013;34(9):554-558

doi:10.3760/cma.j.issn.0254-1785.2013.09.011

Involvement of miR-338-5p in antibody-mediated renal allograft rejection by targeting TRAF3

Haiyan XU ; Xiaozhou HE ; Xuyi MA ; Dong XUE ; Yanyun ZHANG ; Xueguang ZHANG

Keywords

Kidney transplantation; Antibody; Rejection; microRNA

Country

China

Language

Chinese

Abstract

Objective To explore the significantly differentially.expressed microRNAs during antibody-mediated renal allograft rejection.Method MicroRNA array assay was used,and the obtained data were analyzed by bioinformatics analysis.The obtained significant microRNAs were further analyzed to forecast the targeted genes in the common database,then experimental means were used to testify the targeted genes.Result During the antibody-mediated renal allograft rejection,the significantly over-expressed microRNAs were miR-200c,miR-200b,miR-30c,miR-30b and miR-30e+,etc.The significantly down-expressed microRNA was miR-338-5p.The bioinformatics analysis results indicated that TRAF3 was the targeted gene of miR-338-5p,which was testified by real time PCR,immunohistochemical assay and fluorescence reporter assay.Conclusion miR-338-5p anticipated in the antibody-mediated renal allograft rejection by targeting TRAF3.