Chinese Journal of Tissue Engineering Research 2013;(32):5813-5818

doi:10.3969/j.issn.2095-4344.2013.32.011

Mouse embryonic hepatic stem cells differentiate into cardiomyocyte-like cells

Bigang WU ; Jing CHANG ; Xiaogang ZHANG

Keywords

stem cel s; embryonic stem cel s; embryonic hepatic stem cel; dimethylsulfoxide; 5-azacytidine; induction; differentiation; cardiomyocyte-like cel s; α-actin; troponin T; stem cel photographs-containing paper

Country

China

Language

Chinese

Abstract

BACKGROUND:In recent years, embryonic hepatic stem cel s have attracted more attention, but there are few reports on the potential of embryonic hepatic stem cel s to differentiate into cardiomyocyte-like cel s as wel as the related differentiation conditions. OBJECTIVE:To investigate the moderate condition to induce mice embryonic hepatic stem cel s to differentiate into cardiomyocyte-like cel s in vitro with chemical reagents. METHODS:Dimethylsulfoxide in combination with 5-azacytidine with different concentrations and time were used to induce the embryonic hepatic stem cel s of 13.5 days mice and to observe the differentiation effect. RESULTS AND CONCLUSION:Under in vitro conditions, 0.8%dimethylsulfoxide+5μmol/L 5-azacytidine could induce the mouse embryonic hepatic stem cel s to express the specific markers of myocardial cel s, while increasing the concentration of the inducer and extending the induction time could not improve the induction efficacy.