Immunological Journal 2001;(2):132-134

doi:10.3969/j.issn.1000-8861.2001.02.017

Insulin facilitates glucose transporter 4 gene expression in canine heart in vivo

Renfu YIN ; Jinming CHEN ; Zonggui WU ; Shaohua QIU ; Yongmei WANG ; Ruimei WU ; Xiantao KONG

Country

China

Language

Chinese

Abstract

Objective　To investigate the mechanism for that insulin facilitates increase of glucose uptake. Methods　The expression of myocardial GLUT4 polypeptide was determined by semiquantitative immunoblotting. The expression of GLUT4 mRNA was determined by semiquantitative Northern blotting. Results　After infusing insulin for 8　hours，the　expression　of　GLUT4 mRNA and GLUT4 polypeptide was significantly higher in canine myocardium than in　those　found normal ones. The glucose uptake was upregulated at　the　same　time．Conclusions　Our findings suggest that insulin facilitates the expression of GLUT4 mRNA and GLUT4 polypeptide in canine hearts. Enhanced GLUT4 expression is one of the important molecular mechanism by which myocardial cells enhance glucose uptake by insulin stimulation.