OBJECTIVE　To establish a quality standard for Liyan granule.METHODS　Honeysukle flower in the granule was identified by TLC.The developing agent was the upper solvent of butyl acetate formil acid-water(7∶2.5∶2.5).The dual wavelength spectrophotometry was applied for the determination of chlorogenic acid in granule.And the acute toxicity test was accomplished.RESULT　The TLC for the identification of Honeysule flower in the granule was accurate.The uv for chlorogenic acid was reliable.The linear range of chlorogenic acid was 0.46～46.00 μg*mL-1(r=0.9992),and the recovery was 97.33%,RSD=1.49%.The acute toxicity test showed that the maximal tolerable dose in rats was equivalent to 850 times of the clinical dosage in human.CONCLUSION　This method could be used as the quality control during the production of Liyan granule.