Journal of International Pharmaceutical Research 2012;(2):149-153

doi:10.3969/j.issn.1674-0440.2012.02.010

Establishment of LC-MS/MS method for the determination of forsklin in rat plasma and its pharmacokinetics

Dianwei SONG ; Decai WANG ; Zhiyun MENG ; Ruolan GU ; Meihui SHI ; Zhuona WU ; Jingze WANG ; Guifang DOU

Keywords

forsklin; LC-MS/MS; pharmacokinetics

Country

China

Language

Chinese

Abstract

Objective To develop a sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the determination of forsklin in rat plasma.Methods After extraction with methyl tert-butyl ether,chromatographic separation was performed on a C18 column with the mobile phase consisting of water ( 0.1％ formic acid)-acetonitrile in a gradient elution mode.A tandem mass spectrometer equipped with electrospray ionization (ESI) source was used as detector in the positive ion mode.Quantification was performed using multiple reaction monitoring (MRM) with the precursor product combination ions of m/z 411→375.3 and 285→193 for forsklin and diazepam.Results Good linearity was obtained in the 0.5-1000 ng/ml range for the analyte and the analytical method was validated in terms of specificity,precision,accuracy,recovery,stability and matrix effect.These assays gave RSD values always lower than 14.4％ and RE values between -3.5 ％ and 3.8％.In addition,the specificity,extraction recovery,stability and matrix effect were satisfactory.Conclusion Due to its high sensitivity,specificity and simplicity,the method could be used for pharmacokinetic studies of forsklin.