Chinese Journal of Zoonoses 2010;(1):65-68

doi:10.3969/j.issn.1002-2694.2010.01.016

Genetic analysis on the main virulence genes in the Fujian strain of Streptococcus suis type 2 from slaughtered pigs

Qinmei FANG ; Shaoqian HUANG ; Fusong YU ; Jichang ZHU ; Tianlong LIN

Keywords

Streptococcus suis type 2; Fujian strain; virulence-associated gene

Country

China

Language

Chinese

Abstract

To investigate the distribution of virulence associated factors of 5 strains of Streptococcus suis type 2 from slaughtered pigs isolated in Fujian province , 7 virulence genes were detected through PCR amplification. They were the glutamate dehydrogenase gene(gdh), the capsular polysaccharides gene(cps), the extracellular factor gene(ef), the muramidate-released protein gene(mrp), the suilysin gene(sly), the fibronectin-binding protein gene(fbps) and the virulence-associated sequence or f2. Among these 5 strains isolated, the virulence genotypes of 4 strains S.suis type 2 and strain SS2PFJ07 was gdh+/cps2J+/mrp+/ef-/sly-/fbps+/orf2+ and the other one strain isolated from slaughtered pigs was gdh+/cps2J+/mrp+/ef+/sly+/fbps+ /orf2+ respectively. This results showed that there are at least 2 virulence genotypes in Streptococcus suis type 2 from slaughtered pigs isolated in Fujian province.