Chinese Journal of Cancer Biotherapy 2000;7(4):282-284

Construction of Mutant N-ras/61 Gene Recombinant Vaccinia Virus

Jian ZHANG ; Yueran ZHAO ; Li YOU ; Chunyi GAO ; Zhigang TIAN

Keywords

vaccinia virus; N-ras gene; mutant; vaccine

Country

China

Language

Chinese

Abstract

Objective: To construct the recombinant vaccinia virus of mutant N-ras/61 gene and enhance the immunogenecity of mutant N-ras／61 protein produced by the recombinant vaccinia virus. Methods: N-ras／61 gene was inserted into P1108 and transfected into CV-1 cell infected with vaccinia virus by Cell FECTIN. PCR and Western blot were used to identify the recombinants. Results: We get recombinant vaccinia virus rV-N-ras/61 by PCR and tk- selecting. The expression of N-ras gene was detected by Western blot. Conclusion: This study is a test for studing effective vaccine of mutant N-ras/61 gene. The efficacy in vivo of the N-ras／61 vaccine in immunotherapy is under investgation.