Chinese Journal of Cancer Biotherapy 2000;7(4):288-290

Localization Effect of 131 I-Human Anti-HBs Fab in Nude Mice Models of Human Hepatoma

Guichen WU ; Rongcheng LUO ; Huanxing HAN ; Changxuan YOU ; Xuemei DING ; Aimin LI ; Chuanbin WANG ; Mingjang ZHANG

Keywords

hepatoma; nude mice; radioimmunoimaging; HBsAg; monoelonal antibody

Country

China

Language

Chinese

Abstract

Objective: To evaluate the targeting activity in the animal model with human hepatoma, the 131I-human antiHBsAg Fab radioimmunoimaging was explored. Methods: Radioimmunoimagings were taken on different intervals after injection of 131 I-human anti-HBsAg Fab to the nude mice and tissue distribution was measured. The human anti-HBsAg Fab was compared with the murine monoclonal antibodies. Results: The experimental group developed tumor positive images after 3 days of radio-labeled monoclonal antibodies injection, and the peak accumulation of radio-activity on the 5th day.Statistics indicated the tumor/liver ratio of the human anti-HBsAg Fab, murine monoclonal antibodies and the control groups were 5.4,4.0 and 0.9 respectively on the 7th day. Conclusions: Our results suggest that the 131 I-human anti-HB-sAg Fab has a considerable targeting activity, and provide an evidence that it can be used as a novel humanized carrer for targeting therapy of hepatoma.