Chinese Pharmacological Bulletin 2001;17(2):167-170

Effect of Ganoderma polysacchride on intracellular free calcium and intracellular pH in murine T cells

Mingchun LI ; Linsheng LEI ; Qingbiao WANG ; Dongsheng LIANG ; Ziming XU ; Shuqin YANG ; Lisha SUN

Country

China

Language

Chinese

Abstract

AIM　To extend the approach of the act ion of ganoderma polysacchride on intracellular signal events in T cells. METHODS　Laser scanning confocal microscope imaging of the calcium and p H fluorescent indicator dye Fluo-3/AM and SNARF-1/AM were used to determine th e kinetic changes of ［Ca2+］i and ［pH］i in murine T cells induced b y a ganoderma polysacchride,designated GLB7. RESULTS　It was fou nd that GLB7(20 mg*L-1) could increase ［Ca2+］i and ［pH］i at 1 min were 334.7%±16.4%(n=3)、171.6%±10.4%(n=3) respectively. T he increase in ［Ca2+］i induced by GLB7 was due to the influx of extr acellular Ca2+ and intracellular Ca2+ release through both IP3-se nsitive and IP3-insensitive Ca2+ stores, and increase in ［pH］i indu ced by GLB7 was relative to Na+/H+ exchange systems and ［Ca2+］i. GLB 7 did not influence ［Ca2+］i and ［pH］i in murine T cells induced by Con A(3 mg*L-1). CONCLUSION　Stimulation of the increase in ［Ca2+］i and ［pH］i may be an important channel for gano derma polysacchrides to achieve their pharmacological actions.