PurposeTo investigate the permeation of model drug,propranolol hydrochloride, through the excised porcine buccal mucosa. To study the influences of various factors on the buccal absorption also studied. MethodsUsing excised porcine buccal mucosa permeation test in vitro. By means of changing the medium pH, osmotic concentration, drug concentration, the concentration of various penetration enhancers including sodium glycodeoxycholate (GDC), sodium glycocholate (GC), dimethy1-β-cyclodextrin (DMβCD), and hydropropyl-β-cyclodextrin(HPβCD). The permeation rates and permeability coefficients(P) were determined and evaluated. The solubilities and apparent partition coefficients also determined in some tests. ResultsThe result showed that the P were increased when porcine buccal mucosae were pretreated with every penetration enhancement of certain concentration, increased as the pH of test solution rose from 4.9 to 9.1. The P remained constant as the concentration of propranolol hydrochloride increased from 5 to 80 mg/ml, decreased with the increasing of osmotic concentration Conclusions The absorption of propranolol hydrochloride through porcine buccal mucosa is a typical passive process, and affected by penetration enhancers, medium pH, osmotic concentration.