Journal of Peking University(Health Sciences) 2001;33(2):150-152

Oxidized low density lipoprotein stimulated endothelial nitric oxide by activating lectin-like oxidized-LDL receptor

Yaqin XU ; Junhua ZHANG ; yang KE ; jing CAO ; Tao NING ; Liya FENG ; Chaoshu TANG

Country

China

Language

Chinese

Abstract

Objective:To explore the role of lectin-like oxidized low density lipoprotein (ox-LDL) receptor(LOX1) in ox-LDL stimulating endothelial nitric oxide(NO) production. Methods: LOX1 mRNA expression was detected by reverse transcription-polymerase chain reaction(RT-PCR). The quantity of NO was detected by Enzyme-method. Results: Incubation of ox-LDL increased endothelial NO production and LOX1 mRNA expression. When HUVECs were incubated with ox-LDL as well as the inhibitor of LOX1, polyinosinic acid,the increase of NO production and LOX1mRNA expression were attenuated. Conclusion: Ox-LDL enhanced endothelial NO production in a concentration-dependent manner, and the effect of ox-LDL on endothelial NO was mediated by LOX1.