Microbiology 2001;28(2):22-26

ISOLATION AND IDENTIFICATION OF ATRAZINE-DEGRADING STRAINS

Baoli CAI ; Jinyong HUANG ; Jiandang SHI ; Xinping ZHANG ; Hai LIU ; Changshou ZHU

Country

China

Language

Chinese

Abstract

Six atrazine-degrading strains, Pseudomonas spp. AD1, AD2, AD6, Agrobacterium sp. AD4, Xanthomonas AD5, and Erwinia sp. AD7, were isolated from industrial wastewater. These strains are able to grow on atrazine as sole nitrogen source. Strain AD1 is able to degrade atrazine of 0. 3g/L in minimal medium at a percentage of 99.9% in 72 hours. PCR products that are homologous to the atrazine chlorohydrolase gene atzA)from Pseudomonas sp. strain ADP were obtained by PCR method using total DNA of the strains AD1 ,AD4,AD5,AD6,and AD7 as templates.