An analytical method for determination of tetramethylpyrazine (TMP) in ligusticum chuanxiong hort by RP-HPLC coupled with countercurrent extraction used for the preconcentration of trace TMP was established. The column was packed with Zorbax ODS (250 mm×4 mm，5 μm). The mobile phase consisted of methanol water (1 % acetic acid)(35∶65)，the flow rate was 0.8 ml/min and detection was performed at 300 nm. The calibration curves showed good linearity over the range of 0.41 μg/ml to 4.1 μg/ml，γ＝0.9993，the recoveries were 92.3 %. The method was simple，fast，sensitive and accurate and could be applied to the analysis of ligusticum chuanxiong hort. The results showed that the content of TMP in ligusticum chuanxionghort were different with different sources.