Journal of Xinxiang Medical College 2002;19(2):77-80

Substance P inhibits GABA-activated currents in bullfrog primary sensory neurons by protein kinase C

Kaihui YAO ; Junfang WU ; Mingli JI ; Zhibin QIAN ; Yunlei YANG

Keywords

sensory neuron; protein kinase C; substance P; γ-aminobutyric acids; whole-cell patch-clamp technique; bullfog

Country

China

Language

Chinese

Abstract

Objective To research the mechanism or pathway through which Substance P(SP) inhibits r-aminobutyric acid(GABA) activated currents in bullfrog dorsal root ganglion(DRG) neurons.Method The experiment was conducted on freshly isolated bullfrog dorsal root ganglion neurons using a whole-cell patch-clamp technique.Results SP could caused a slow inward current when SP was applied to DRG neurons;SP could inhibits GABA-activated currents;The inhibition could be reduced largely when protein kinase C(PKC) inhibiter,1-(5-isoquinolinesulphorry)-2-methylpiperazine(H-7), was dialyzed in cell body.Conclusion The SP ton inhibit GABA-activated currents through protein kinase C.