Acta Anatomica Sinica 2005;36(3):320-325

A UNIVERSAL PRIMER U2 LABELING METHOD FOR MICROARRAY ANALYSIS

Shuyan WANG ; Wenling ZHENG ; Jueyu ZHOU ; Dapeng DING ; Qiulin XU ; Haiyan ZHANG ; Yifei PENG ; Rong SHI ; Wenli MA

Keywords

Universal primer labeling(UPL); Influenza virus; Microarray; Labeling

Country

China

Language

Chinese

Abstract

Objective To report a new method of fluorescent labeling technique in microarray studies: universal primer U2 labeling( UPL). The efficiency was compared of the UPL with that of random primer, restriction display labeling method and the reverse transcription coupled random primer spiking labeling method(RT-PSL). Methods Influenza viral RNA was labeled with both UPL and the conventional random primer labeling method as well as two other more laborious labeling methods( RD-direct and RD-incorporate), and hybridized with influenza virus oligonucleotide microarrays. The signals extracted from the microarrays were analyzed using SPSS 10.0 software. Results The fluorescent intensity, signal-to-noise ration(SNR), true positive ratio(TPR) of probes and labeling reproducibility of UPL were demonstrated to be higher than those of the Random primer approaches.Conclusion These results established that UPL is a valid new labeling protocol, which may have wide applications in the research and development of the microarray technology.