Chinese Traditional Patent Medicine 2005;27(4):424-426

Determination of tanshinone Ⅱ A in Compound Dangguifukang Capsule by RP-HPLC

Xinchun WANG ; Zhigeng YU ; Weijun CHEN ; Zhilin HU

Keywords

tanshinone Ⅱ A; Dangguifukang Capsule; HPLC

Country

China

Language

Chinese

Abstract

AIM: To establish a HPLC method for the determination of tanshinone Ⅱ A in Compound Danguifukang Capsule. METHODS: Analytic column: Reliasil C18(5μm, 250mm×4.6mm), protective column: (5μm, 12.50mm×4.6mm); mobile phase: methnaol-ultrapure water (80: 20); detective wavelength was at 270a good linearity within the range of 1×10-2-0.16μg, r=0.9995. The average recovery was 99.73% and RSD was 2.91%. CONCLUSION: The method is feasible, and simple to operate and suitable for the quality control of Compound Dangguifukang Capsule.