Journal of Xinxiang Medical College 2006;23(6):541-543

Activation of nuclear factor kappaB of monocyte in patients with chronic obstructive pulmonary disease

Yong QI ; Yuepeng GUO ; Xiaomei YUAN ; Jinlan LI ; Lijun MA

Keywords

chronic obstructive pulmonary disease; nuclear factor-κB; interleukin-8

Country

China

Language

Chinese

Abstract

Objective To investigate the role of NF-κB activation in the inflammatory mechanism of chronic obstructive pulmonary disease (COPD).Methods Monocyte were collected from patients with COPD and were cultured,and stimulated with lipopolysaccharide (LPS) ; NF-κB p65 activation was measured by immunohistochemistry; SOD ,MDA and IL-8 and lung function were determined synchronously.Results The NF-κB p65 was induced by LPS in monocyte in all subjects, but it was most markedly done in COPD patients with exacerbateions; There was positive correlation between the NF-κB p65 activation of monocytes and levels of IL-8 and MDA in circulation, but it was negative correlation to SOD.Conclusion NF-κB plays a vital role in regulating product of IL-8 in monocyte in COPD.