Chinese Journal of Neuroanatomy 2008;24(1):8-12

Morphological alterations induced by didanosine in cultured dorsal root ganglion nrurons

Huaxiang LIU ; Zhen LIU ; Xiangdong YANG ; Lihong WANG ; Zhenzhong LI

Keywords

didanesine; dorsal root ganglion; neuron; rat

Country

China

Language

Chinese

Abstract

To investigate the effects of didannsine(ddI)on the morphological alterations of dorsal root ganglion(DRG)neurons,dissoci-ated DRG cells from rat embryo were studied.DRG cells were cultured for 3 days and then treated with ddI for additional 3 claysin differ-ent concentrations(1μg/ml,5 μg/ml,10μg/ml and 20 μg/ml,respectively).Afarthat,DRG cells were processedformicrotubule as-soeiated protein 2(MAP2)labeling and observed under confocal laser scanning microscopy(CLSM).The results showed that both thenumber and length of neurites of the DRG cells after exposed to ddl significantly down-regulated in a dose-dependentmanner compared withcontrol group,thus suggesting that ddI may have inhibitory effects on neufite regeneration and outgrowth in dissociated DRG cultures.