Chinese Journal of Biochemistry and Molecular Biology 2008;24(8):712-718

Comparative Proteome Analysis of Plasma Membrane from Different Differential Nasopharyngeal Carcinoma Cell Lines

Tingting SHENG ; Lijun ZHANG ; Xiaohui LIU ; Xiaofang JIA ; Jixian XIONG ; Quanyuan HE ; Rui CAO ; Xia PENG ; Ni SHI ; Songping LIANG

Keywords

nasopharyngeal carcinoma; differentiation; plasma membrane; nlass spectrometry; proteome

Country

China

Language

Chinese

Abstract

A subcellular proteomic method was applied to investigate the protein expression profiles of nasopharyngeal carcinoma (NPC) cell lines,CNE1 and CNE2,at various differentiation levels.Plasma membrane (PM) proteins were obtained by Percoll density grade centrifugation and subjected to twodimensional electrophoresis (2DE) followed by PDQuest software analysis.Nine proteins expressed with more than two folds difference were identified by MALDI-TOF-TOF,of which functions involved in cell differentiation,signal transduction,and metabolism.Half of these proteins,such as galectin-1 and annexin Ⅱ,were analyzed with real-time quantitative PCR or Westem blotting.We have tested a proteomic method to study differentiated NPCs at different levels and found several proteins that might be related to their biological characteristics.