Chinese Journal of Veterinary Science 2009;29(7):924-927

Effects of recombinded resistin on pyruvate carboxylase mRNA expression and activity in culture bovine hepatocyte in vitro

Aodi CHEN ; Pengfei HE ; Guowen LIU ; Chengzhi CHEN ; Zhe WANG

Keywords

recombinded resistin; hepatocyte; pyruvate carboxylase; calf

Country

China

Language

Chinese

Abstract

A single factor duplicate test was designed to investigate whether bovine recombinded resistin impacts the expression of pyruvate carboxylase (PC) mRNA and the activity of PC in vitro culture bovine hepatocyte.Bovine recombinded resistin was added to the media with 0,25,50,100,200 and 400 ng/L.Abundance of PC mRNA in bovine hepatocyte,which was cultured with bovine recombinded resistin for 12 hours,was determined by real-time fluorescence quantitative RT-PCR,and activity of PC was determined by colourimetry.The results showed that bovine recombinded resistin could downregulate the expression of PC mRNA and the activity of PC in vitro culture bovine hepatocyte.