Acta Medicinae Universitatis Scientiae et Technologiae Huazhong 2010;39(1):82-86

doi:10.3870/j.issn.1672-0741.2010.01.020

Involvement of Heme Oxygenase-1 in the Protective Actions of Curcumin against Ethanol-induced Oxidative Damage in Rat Primary Hepatocytes

Yingying OUYANG ; Ke LI ; Shuang RONG ; Ping YAO ; Nianhong YANG ; Xiufa SUN ; Liegang LIU

Keywords

curcumin; heme oxygenase-1; ethanol; oxidative stress

Country

China

Language

Chinese

Abstract

Objective To investigate the role of heme oxygenase-1(HO-1)in the protective actions of curcumin against ethanol-induced oxidative damage.Methods Microsomal HO-1 enzyme activities were determined in rat primary hepatocytes pretreated by curcumin.AST,LDH release and hepatic oxidative/antioxidant status were measured with or without ZnPPⅨ/Hemin as a classic HO-1 inhibitor/inducer,respectively.Results Curcumin could induce the HO-1 expression and enzyme activity,which was correlated with antioxidant levels in hepatocytes.HO-1 induction reached a peak under administration of 15 μmol/L curcumin for 1 h.Conclusion HO-1 induction by curcumin is contributed to the heptoprotective effects against ethanol-induced oxidative damage.