Chinese Journal of Nervous and Mental Diseases 2010;36(3):129-132

Knockdown of EphB4 gene by small interference RNA inhibits proliferation of glioma cells in vitro

Chunhui LI ; Zhihong LI ; Yanfang SHI ; Yi GUO ; Yanan DING ; Haipeng LIU ; Kai CUI

Keywords

siRNA; EphB4; Glioma

Country

China

Language

Chinese

Abstract

Objective To explore the role of EphB4 in proliferation of glioma cells. Methods The mRNA and protein expressions of EphB4 were detected using RT-PCR, immunochemistry, and Western-blot, respectively. EphB4 siRNA was synthesized and transfected into U251 cells using Lipofectamine 2000. Glioma cell proliferation, apoptosis, and invasion were determined by MTT assay, TUNEL and transwell experiment, respectively. Results The expression (P<0.05) and proliferation of EphB4 were obviously decreased in U251 transfected with EphB4 siRNA and the proliferation was further decreased with the increased concetrations of siRNA. Compared with U251 group and siRNASCR group, EphB4 siRNA at different concentrations (25, 50 or 100 nmol/L) significantly reduced the invasion ability of cells and increased the number of apoptotic cells (P<0.05). Conclusions EphB4 plays an important role in the regulation of glioma cell proliferation, apoptosis and invasion.