Objective To observe the effect of treating neonatal jaundice with Qing Kai Ling injection plus photoradiation. Methods 583 cases were randomly recruited into a control group and a treatment group.342 cases in the treatment group received Qing Kai Ling injection plus photoradiation treatment, while 241 cases in the control group received only photoradiation treatment. TBIL, DBIL regression time of jaundice and symptom improvement were observed in both groups. Results After the treatment, TBIL and DBIL's level in the treatment group was lower than the control group (P< 0.05) . symptoms improving in the treatment group was also significantly better (P=0.0087) . The recovery time for the treatment group was shorter than the control group (P= 0.0068). Conclusion Qing Kai Ling injection plus photoradiation is good in treating neonatal jaundice.