Chinese Journal of Urology 2010;31(10):707-709

doi:10.3760/cma.j.issn.1000-6702.2010.10.017

Study on histone acetylation modulation and Akt signaling pathway inhibition by phenyhexyle isothiocyanate in prostate cancer PC3 cell line

Zhiming ZHUANG ; Xudong MA ; Yiqun HUANG ; Zhouda ZHENG ; Yacai ZHENG ; Shaohong JIANG

Keywords

Phenyhexyle isothiocyanate; Prostatic neoplasms; Akt signaling pathway; Histone; Acetylation

Country

China

Language

Chinese

Abstract

Objective To investigate phenyhexyle isothiocyanate (PHI) modulating histone acetylation and inhibiting Akt signaling pathway in prostate cancer cell line PC3 in vitro. Methods Apoptotic cells were measured by TUNEL assay. Histone acetylated H3, H4 and the Akt protein signaling pathway (Akt, p-Akt, mTOR, p-mTOR, p70S6K and p-p70S6K) were detected by Western blot. Results Apoptotic cells increased after exposure to PHI with concentration dependent. PHI significantly induced an accumulation of histone acetylated H3, H4. The change of Akt, mTOR, p70S6K proteins was not observed. Phosphorylation of Akt (p- Akt), mTOR (p-mTOR) and p70S6K (p-p70S6K) decreased after exposure to PHI for 7 h. Conclusions PHI can induce histone acetylation H3, H4 accumulation. PHI inhibits Akt signaling pathway resulting cell apoptosis. It might be a new anticancer agent.