Objective To induce islet grafting tolerance by intravenous injection of anti-CD4,anti-CD8 immunotoxins and donor soluble antigen.Methods 14 days or 7 days prior to transplantation,the immunotoxon 200 μg respectively,and donor soluble antigen 500 μg were injected intravenously into the recipients, then 500 donor islets were translanted under the left renal subcapsular space of diabetes reciPients (SD rats).Results The islet grafting survival time that pretreated with immunotoxon and dono soluble antigen was over 60 days(P<0.01).The immunotoxins or donor soluble antigen treatment alone only slightly prolonged the graft survival.Conclusion The anti-CD4,anti-CD8 immunotoxins combined with donor soluble antigen can induce donor specific immune tolerance.