Chinese Journal of Radiological Medicine and Protection 2010;30(6):658-660

doi:10.3760/cma.j.issn.0254-5098.2010.06.008

Comparison of 3H-TdR and 125Ⅰ-UdR incorporation on the proliferation effect of lymphocytes

Jianmei WAN ; Youjiu ZHANG ; Wo FAN ; Ran ZHU ; Ping NING ; Fengmei CUI

Keywords

Lymphocytes; Lymphoma cells; 3H-TdR; 125Ⅰ-UdR; Incorporating fraction

Country

China

Language

Chinese

Abstract

Objective To compare the incorporation method of 3H-TdR and 125Ⅰ-UdR on determining the proliferation effect of lymphocytes. Methods The proliferation effects of lymphocyte and Daudi lymphoma cells were estimated by 3H-TdR and 125Ⅰ-UdR incorporation. Results The incorporating fraction of 3H-TdR and 125Ⅰ-UdR into lymphocyte was 20.95% ± 1.06% and 1.00% ±0.04%,respectively, and the incorporating fraction for the lymphoma cells was 29. 94% ± 4. 10% and 6. 02% ±0. 73% ,respectively. The incorporation fractions of 3H-TdR into lymphocyte and lymphoma cells were much higher than those of 125Ⅰ-UdR, but the incorporating fractions of 3H-TdR or 125Ⅰ-UdR into the lymphoma cells were much higher than those of lymphocytes. Conclusions For lymphocytes, 125Ⅰ-UdR cannot substitute 3H-TdR as a tracer agent. But for lymphoma cells, whether 125Ⅰ-UdR could be replace 3H-TdR or not needs further research.