Chinese Journal of Neurology 2011;44(3):159-162

doi:10.3760/cma.j.issn.1006-7876.2011.03.003

The study on the role of transcription factor GATA binding protein 3 in familial Alzheimer's disease pathogenesis

Wei QIN ; Aihong ZHOU ; Xiumei ZUO ; Fen WANG ; Zhe CHENG ; Jianping JIA

Keywords

Alzheimer disease; Presenilin-1; Insulin degrading enzyme; GATA3 transcription factor

Country

China

Language

Chinese

Abstract

Objective To investigate the mechanisms of decreasing insulin degrading enzyme (IDE) level by mutation V97L in the gene presenilin 1 (PS-1).Methods Transcription factor GATA binding protein 3 (GATA-3) activity was assessed by protein/DNA array and verified by Western blot in SH-SY5Y cells transfected by PS-1 mutation V97L.Results Protein/DNA array and Western blot revealed that there was increased transcript factor activity (5.5 times high) and protein level of GATA-3 in V97L-PS-1 transfected SH-SY5Y cells.Transcription factor GATA-3 can bind to the IDE promoter and negatively control the IDE transcription level.Conclusion PS-1 mutation V97L may regulate the transcription of IDE via GATA-3, and subsequently involve in deposition of Aβ42 and development of Alzheimer's disease.