Cancer Research and Clinic 2010;22(11):726-729

doi:10.3760/cma.j.issn.1006-9801.2010.11.003

Antitumor effect on gastric cancer in vitro of selective replicative-competent adenovirus CNHK300-mIFN-γ

Jian CHEN ; Xushun LIU ; Feng WANG ; Guangquan ZONG ; Changqing SU ; Linfang LI ; Lihua JIANG

Keywords

Stomach neoplasms; Replicative-competent adenovirus; Gene therapy; mIFN-γ

Country

China

Language

Chinese

Abstract

Objective To compare the expression of mIFN-γ, replicative activities and anti-tumor activities of CNHK300-mIFN-γand Ad-mIFN-γin normal and gastric cancer cells. Methods The replicative activities of viruses in cells were measured by viral replication assay. CPE assay was used to detect the antitumor effect of viruses. The expression level of mIFN-γ in cancer cells was detected by ELISA. Results The infection of CNHK300-mIFN-γled to an obvious expression of mIFN-γin gastric cancer cells. The vector system CNHK300-mIFN-γpossessed more replicated potential than Ad-mIFN-γ, and could specifically kill most of BGC-823 cells at MOI value of 0.1, which was much better than that by the traditional adenoviral vector. Conclusion CNHK300-mIFN-γcan selectively replicate and effectively express mIFN-γ in tumor cells, and specifically kill gastric cancer cells, suggesting a splendid future as a new anticancer agent.