Virologica Sinica 2011;26(1):61-66

doi:10.1007/s12250-011-3154-9

Indirect ELISA with Recombinant GP5 for Detecting Antibodies to Porcine Reproductive and Respiratory Syndrome Virus

Yan CHEN ; Hong TIAN ; Jianhui HE ; Jinyin WU ; Youjun SHANG ; Xiangtao LIU

Keywords

Porcine reproductive and respiratory syndromevirus(PRRSV); GP5 protein; Indirect ELISA

Country

China

Language

Chinese

Abstract

Porcine reproductive and respiratory syndrome is caused by the PRRS virus(PRRSV), which has six structural proteins(GP2, GP3, GP4, GP5, M and N). GP5 and N protein are important targets for serological detection by enzyme-linked immunosorbent assay(ELISA)and other methods. Toward this goal, we developed an indirect ELISA with recombinant GP5 antigens and this method was validated by comparison to the LSI PRRSV-Ab ELISA kit. The results indicated that the optimal concentration of coated recombinant antigen was 0.2 μg/well for a serum dilution of 1:40. The rate of agreement with the LSI PRRSV-Ab kit was 88.7%(266/300). These results support the potential use of recombinant GP5 as an antigen for indirect ELISA to detect PRRSV antibodies in pigs.