Japanese Journal of Complementary and Alternative Medicine  2017;14(1):33-37

doi:10.1625/jcam.14.33

Safety Evaluation of Methoxyflavones Mixture (Ⅱ) from Kempferia parviflora：28-day Repeated Dose Oral Toxicity Test

Hirotaka OHKUWA-HAYASHI ; Takanori FUJITA ; Takuya KAWATA ; Yoshihisa NAKANO ; Tomihisa OHTA

Keywords

Kaempferia parviflora; methoxyflavone; 28-day repeated dose oral toxicity test

Country

Japan

Language

Japanese

Abstract

Kaempferia parviflora rhizome is used folk medicines for treatment of various symptoms in Thailand since anticent times.Several types of methoxyflavones has been identified in this plant and their physiological functions have been reported.We determined that six kinds of methoxyflavones (5,7,3',4'-tetramethoxyflavone, 3,5,7,3',4'-pentamethoxyflavone, 5,7-dimethoxyflavone, 5,7,4'-trimethoxyflavone, 3,5,7-trimethoxyflavone, 3,5,7,4'-tetramethoxyflavone) were included in the 80% ethanol extract of K. parviflora rhizome.The safety of six methoxyflavones mixture was evaluated with 28-day repeated oral dose toxicity test in mice.These results indicated no significant toxicity on body weight, blood analyses, organ weight, blood biochemical analyses.