Korean Journal of Gynecologic Oncology  2005;16(4):347-353

Effects of cell growth inhibition on the combination of cisplatin with green tea extracts.

Hyun Kyung KIM 1 ; Young Haw KANG ; Sun Young KWAK ; Guo Hua DING ; Su Mi BAE ; Eun Kyung PARK ; Yong Seok LEE ; Jung KIM ; Yong Wook KIM ; Duck Yeong RO ; Joon Mo LEE ; Sung Eun NAMKOONG ; Hong Seok CHANG ; Heung Jae CHUN ; Dae Seog LIM ; Woong Shick AHN

Affiliations

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Keywords

Cisplatin; TC-1 cell line; GTP; EGCG

Country

Republic of Korea

Language

Korean

Abstract

OBJECTIVE: The chemotherapeutic agent cisplatin (cis-diamminedichloroplatinum (II)) is particularly effective against cervical cancer. The purpose of this study is to elucidate combination effect of cisplatin and green tea extracts on the growth inhibition of TC-1 cell. METHODS: To observe the anti-proliferative effects, we treated different doses of cisplatin (0.1, 0.5, 2.5 uM), GTP (1, 5, 25 ug/ml) and EGCG (25, 50, 100 uM). to TC-1 cells. Also, we treated 0.5 uM of cisplatin and different doses of GTP (1 and 5 ug/ml) or EGCG (25 and 50 uM). Cell viability was scored by use of MTT assay. In addition, E6 gene expression patterns in TC-1 cell were investigated by using RT-PCR. RESULTS: Cell growth inhibition in a dose dependent was observed at the different concentration of ciaplatin, GTP and EGCG. Also, in the groups treated by 0.5 uM of cisplatin and GTP (1 and 5 ug/ml) or EGCG (25 and 50 uM), the inhibition of cell growth showed with 12.2%, 6.9% and 63.4%, 72.2% as compared to the group treated by cisplatin only. In RT-PCR, down regulation of E6 was shown. CONCLUSION: Additive effect of the combination of cisplatin with GTP or EGCG on the inhibition of cell growth was observed. This effect suggests the possibility lowering the concentration of chemotherapeutic drugs, which alleviate the side effect of drugs.