Chinese Journal of Virology 2012;28(4):414-417

Degradation of 14-3-3beta appeared in apoptosis cell induced by PrP106-126 polypeptide.

Peng SUN 1 ; Juan SONG ; Jin ZHANG ; Qin-Qin SONG ; Xing GAN ; Yu CUI ; Chen GAO ; Xiao-Zhen BO ; Jun HAN

Affiliations

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Country

China

Language

Chinese

Abstract

To investigate changes of 14-3-3beta from apoptosis induced by PrP106-126 polypeptide, HeLa cell was incubated with PrP106-126 for 4h or 8h. Nucleus changes and the expression of PARP were detected differently by Hoechst staining and Western blotting. Expressing of protein and mRNA from 14-3-3beta was determined by Western blotting and Real-time PCR. The results show that typical nucleus pyknosis and chip of apoptosis and degradation of PARP were induced by PrP106-126 peptide in HeLa cells. Degradation of 14-3-3beta appeared in apoptosis groups induced by PrP106-126 peptide. However, 14-3-3beta mRNA did not display any changes in apoptosis groups. This study indicated that degradation of antiapoptosis protein 143-3beta induced by PrP106-126 peptide may be one of pathogenesis mechanism of prion disease.