Experimental & Molecular Medicine  2009;41(8):548-554

doi:10.3858/emm.2009.41.8.060

Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-kappa B pathway in human dermal fibroblasts.

Young Rae LEE 1 ; Eun Mi NOH ; Eun Yong JEONG ; Seok Kweon YUN ; Young Ju JEONG ; Jong Hyeon KIM ; Kang Beom KWON ; Byeong Soo KIM ; Sung Ho LEE ; Chang Sik PARK ; Jong Suk KIM

Affiliations

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Keywords

cordycepin; matrix metalloproteinases; NF-kappa B; skin aging; ultraviolet rays

Country

Republic of Korea

Language

English

Abstract

Cordycepin (3'-deoxyadenosine) has been shown to exhibit many pharmacological activities, including anti-cancer, anti-inflammatory, and anti-infection activities. However, the anti-skin photoaging effects of cordycepin have not yet been reported. In the present study, we investigated the inhibitory effects of cordycepin on matrix metalloproteinase-1 (MMP-1) and -3 expressions of the human dermal fibroblast cells. Western blot analysis and real-time PCR revealed cordycepin inhibited UVB-induced MMP-1 and -3 expressions in a dose-dependent manner. UVB strongly activated NF-kappa B activity, which was determined by I kappa B alpha degradation, nuclear localization of p50 and p65 subunit, and NF-kappa B binding activity. However, UVB-induced NF-kappa B activation and MMP expression were completely blocked by cordycepin pretreatment. These findings suggest that cordycepin could prevent UVB-induced MMPs expressions through inhibition of NF-kappa B activation. In conclusion, cordycepin might be used as a potential agent for the prevention and treatment of skin photoaging.