Korean Journal of Veterinary Research  2014;54(2):113-115

Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces.

Yong Kwan KIM 1 ; Ha Young KIM ; Albert Byungyun JEON ; Myoung Heon LEE ; You Chan BAE ; Jae Won BYUN

Affiliations

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Keywords

detection limit; invA; loop-mediated isothermal amplification assay; Salmonella

Country

Republic of Korea

Language

English

Abstract

Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella infection. The detection limits for broth dilution, spiked feces and enrichment were 10(4), 10(5) and 10(2) CFUs/mL, respectively. The LAMP assay developed in the present study may be a reliable method for detection of Salmonella spp. in pig feces.